Seasonal influenza virus infection is one of the largest recurring global public health threats. None of the above funders had any role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The project described in this publication was also supported by the University of Rochester CTSA award UL1 TR002001 from the National Center for Advancing Translational Sciences of the National Institutes of Health (MZ). įunding: This work was supported by grants from the National Institutes of Health, National Institutes of Allergy and Infectious Diseases, including: HHSN272201000055C, AI098112, and AI069351 (MZ, JW, SH, OH, SP, JG, NH), R21AI138500 (MZ, JW, JG), HHSN272201400008C and AI109946(FC, FA). This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: The data underlying this study have been uploaded to figshare and are accessible using the following link. Received: DecemAccepted: FebruPublished: April 11, 2018Ĭopyright: © 2018 Wang et al. Huber, University of South Dakota, UNITED STATES (2018) Broad cross-reactive IgG responses elicited by adjuvanted vaccination with recombinant influenza hemagglutinin (rHA) in ferrets and mice. We have also found that adjuvant plays a critical role in the generation of long-lived ASCs and MBCs cross-reactive to influenza viruses as a result of vaccination with rHA of influenza virus, and the observed increase in stalk-reactive antibodies likely contributes to this IgG mediated broad cross-reactivity.Ĭitation: Wang J, Hilchey SP, DeDiego M, Perry S, Hyrien O, Nogales A, et al. We also characterized the generation of cross-reactive antibody secreting cells (ASCs) and memory B cells (MBCs) when comparing vaccination to viral infection. Using this same mouse vaccination model, we determined that Addavax plays a more significant role in the initial priming event than in subsequent boosts. We observed a similar phenomenon in mice receiving three sequential vaccinations with rHA proteins from either A/California/07/2009 (H1N1) or A/Hong Kong/1/1968 (H3N2) viruses admixed with Addavax, an MF59-like adjuvant. In the current study, using Luminex-based mPlex-Flu assay, developed by our laboratory, to quantitatively measure influenza specific IgG antibody mediated cross-reactivity, we found that prime-boost-boost vaccination of ferrets with rHA proteins admixed with adjuvant elicited higher magnitude and broader cross-reactive antibody responses than that induced by actual influenza viral infection, and this cross-reactive response likely correlated with increased anti-stalk reactive antibodies.
However, the optimal strategies for achieving highly cross-reactive antibodies to the influenza virus HA have not yet to be defined.
Accumulating evidence suggests that antibody mediated cross-reactive immunity against influenza hemagglutinin (HA) strongly correlates with long-lasting cross-protection against influenza virus strains that differ from the primary infection or vaccination strain. Annual immunization against influenza virus is a large international public health effort.